In the present study utilization of pineapple waste viz pulp, peels and crown leaves for production of acetic acid (vinegar) which is commonly called as vinegar was done. Firstly the production medium was inoculated with Saccharomyces cerevasae procured from active dry yeast. The medium was kept for about seven days for alcoholic fermentation at room temperature. It was found that alcohol percentage for pulp was 3.75%, peels were 2.5% and crown leaves were 6.25%.  After confirming alcohol percentage the medium was inoculated with Acetobacter aceti for acetic acid fermentation and it was incubated for the next 14 days at 37°C. The 9.6%, 10.2% and 10.8% of acetic acid was found to be produced from pulp, peels and crown leaves respectively. As from results it was found that increase in acetic acid concentration decreases pH of the medium. The present study also helpful in conversion of waste into the useful value added product like acetic acid (vinegar).