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VOL. 10, ISSUE 4 (2025)
In vitro seed germination, differentiation and phytochemical screening of the medicinal orchid Acampe ochracea of Bangladesh
Authors
Marzia Akter Sumi, Awishik Tripura, Momena Khanam Mumu, Minhajur Rahman and Tapash Kumar Bhowmik
Abstract
Acampe ochracea
(Lindl.) Hochr. is a medicinally significant epiphytic orchid indigenous to
Bangladesh, currently threatened by habitat degradation and overharvesting.
This study establishes a reproducible protocol for asymbiotic seed germination
and comparative phytochemical profiling to facilitate ex situ
conservation and pharmaceutical utilization. Immature seeds were cultured on
four basal media (MS, PM, MVW and KC) supplemented with (0.5 mg/l BAP and 0.5
mg/l NAA) or without plant growth regulators (PGRs). PM (Phytamax) medium
fortified with PGRs proved superior, yielding the highest germination rate
(73.34%) and the earliest protocorm initiation (9.43 ± 0.25 weeks). Complete
plantlet development, characterized by healthy root and shoot differentiation,
was achieved within 24.33 weeks. Following acclimatization on a coir, charcoal
and brick enriched substrate, seedlings exhibited an 82% survival rate.
Furthermore, qualitative phytochemical screening revealed that in vitro
derived tissues synthesized comparable or superior levels of secondary
metabolites specifically alkaloids and saponins compared to wild counterparts.
These findings confirm the efficacy of the protocol for mass propagation and
suggest that in vitro culture conditions may enhance the biosynthesis of
bioactive compounds, offering a sustainable alternative to wild harvesting for
medicinal applications.
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Pages:133-137
How to cite this article:
Marzia Akter Sumi, Awishik Tripura, Momena Khanam Mumu, Minhajur Rahman and Tapash Kumar Bhowmik "<i>In vitro</i> seed germination, differentiation and phytochemical screening of the medicinal orchid <i>Acampe ochracea</i> of Bangladesh". International Journal of Advanced Scientific Research, Vol 10, Issue 4, 2025, Pages 133-137
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