The present study aimed to isolate and characterize plant growth promoting bacteria (PGPB) from rhizospheric soil collected from the arid region of Bikaner, Rajasthan, India. A total of fifteen morphologically distinct bacterial isolates (BIK1a–BIK3e) were successfully isolated using serial dilution and nutrient agar culture techniques. Preliminary Gram staining revealed that 73.3% of the isolates were Gram-positive, while 26.7% were Gram-negative. Biochemical characterization showed high enzymatic diversity: all isolates (100%) were catalase positive, 86.7% exhibited urease activity, and 80% demonstrated amylase (starch hydrolysis) capability. H₂S production was detected in 46.7% of isolates, whereas 93.3% and 66.7% tested positive for Methyl Red (MR) and Voges–Proskauer (VP) tests respectively, indicating varied fermentative pathways.