Establishment and maintaining cultures from stem node explants of Corallacarpus epigaens (Rottler & willd)
The predominant Corallocarpus epigaens species can survive well in high temperature, slight frost and low rainfall. Their roots penetrate deeply in to ground water level and so they do not compete for water with the crop plants (Leaky and Last, 1980). The situation has become compounded by various inherent biological problems. Although we have modern technologies and fast developing industrial sector, gas and electricity are neither available nor affordable for this large section of the population. The Major critical prerequisite for all transformations procedures is the ability in establishing and maintaining a culture with highly responsive plant tissues. Frequently sub cultured suspension cultures and cells taken from the early log phase are almost suitable Vasil and Vasil (1979). Mohan et al (1998). Plant regeneration culture. Tropical hardwood tree species that has several applications in the lumber industry and is used in agro forestry systems (Galeano et al 2013 & 2015) Most of species are scattered widely throughout tropical and subtropical arid regions. Several of these are categorized as “multipurpose trees” and are backbone of rural economy throughout the drier plants of the world. It is because of the dependence on these species that plants have become over exploited. Tremendous pressure exerted by both man and animal, resulted in complete removal of superior germplasm or in some cases plant species have become threatened (Ramawat and Nadwani, 1999).