Nowadays, the medicinal plants are the good source of remedy for various infections and diseases. It has a potential antibacterial activity against various pathogenic bacteria. In this study, we investigate the antibacterial activity of Ocimumbasilium was determined for the various bacterial strains with aqueous, ethanol and acetone extracts. These studies present the ethanol extract has the maximum inhibitory effect for all the strains and the aqueous extract showed the high inhibitory effect on Pseudomonas aeruginosa. The highest zone of inhibition was recorded for the acetone extracts for Pseudomonas aeruginosa, Staphylococcus aureus and Escherichia coli. These results concluded that the ethanol extract of Ocimumbasillicum has the potential antibacterial activity against pathogenic bacterial strains.