International Journal of Advanced Scientific Research

Authors
Magashi LA, Adamu HM, Kolo AM, Hassan UF

Abstract
The Caffeic acid (3-(3,4-Dihydroxyphenyl)-2-propenoic acid) was isolated from Cassia sieberiana root bark using standard analytical methods and the main compound with [ M + H] mass of 360 m/z with retention time of 0.495 in positive mode and 0.392 in negative mode, by exhibiting the base peak ion at 359 m/z was identified. Dimer of Caffeic acid was isolated and fully characterized with concerted use of FTIR, LCMS, HNMR, ¹³CNMR spectroscopy and CHN analysis. The CHN calculations from the empirical formular C₂H₂O (42 g) and the actual mass of the compound was calculated to be 378 g/mol by comparing molecular weight obtained from MS (360 m/z). C₁₈H18O₉ (378 g/mol) with loss of water molecule (18 g/mol) gave a compound of mass 360 g/mol (C₁₈H₁₆O₈) which is the dimer of caffeic acid 180 g (C₉H₈O₄) (39) and subsequence loss of water gave the actual mass of dicaffeic acid (C₁₈H₁₄O₇) (38) are in agreements with experimental results confirmed the identity of the isolated natural product.