International Journal of Advanced Scientific Research

International Journal of Advanced Scientific Research


International Journal of Advanced Scientific Research
International Journal of Advanced Scientific Research
Vol. 5, Issue 4 (2020)

Evaluation of genetic polymorphism of interleukin-1β +3954 (rs1143634) in chronic & aggressive periodontitis in Bengali population of West Bengal, India


Avishek Das, Ritam Kundu, Debajyoti Mondal, Ashit Kumar Pal, Somen Bagchi

Background & Objective: The genetic basis of periodontitis was demonstrated by formal genetic studies which were focused on a range of various candidate genes selected for their roles in the immune system like genes of the Interleukins (IL) which regulate the intensity of host immune-inflammatory response. This regulation of host response may be associated with the genetic polymorphisms, specifically single nucleotide polymorphisms of the genes of various Interleukins. Interleukin-1(IL-1) is a principal mediator of inflammatory responses acting on many cell types and is itself produced by many different cells, including macrophages, endothelial cells, B cells, fibroblasts, epithelial cells, astrocytes and osteoblasts in response to microorganisms, bacterial toxins and complement components. In the present study, attempt has been made to explore the role of IL-1β+3954 (rs1143634) gene polymorphism in Chronic as well as Aggressive Periodontitis in Bengali population of West Bengal, India. Materials & Methods: Total 88 Bengali patients of both sex were recruited in this study and they were divided into 3 groups: Group A (Chronic Periodontitis group), Group B (Aggressive Periodontitis group) and Group C (Healthy control). The clinical parameters taken into consideration for the assessment of chronic and aggressive periodontitis were Plaque index, Calculus index, Gingival index, Probing pocket depth (PPD), Clinical attachment loss (CAL). 3 ml of peripheral venous blood was collected from each selected participants & transferred to a 3% EDTA containing serum vial and stored at -20°C for DNA extraction. DNA extraction was performed by phenol chloroform method & ethanol Precipitation. Genotyping of extracted DNA samples was carried out for locus IL-1β+3954 (rs1143634) by Real – time Polymerase Chain Reaction. Hardy–Weinberg equilibrium was tested for all the gene polymorphisms and association between genotypes and cases was examined by Odds ratio with 95% confidence interval (CI) and chi-square analysis using R statistical software. Allelic frequencies were calculate according to the number of different alleles observed and the total number of alleles examined. Statistical significance was defined as p < 0.05. Results: Upon analysis of minor allele frequencies of total periodontitis cases and control, the results were found to be statistically insignificant with the p value of 0.7036. Conclusions: The present study suggested a no association of single nucleotide polymorphism of IL-1β+3954 (rs1143634) with total periodontitis cases (both Chronic Periodontitis and Aggressive Periodontitis) in Bengali population of West Bengal, India.
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