The micronutrients and their requirements for proper growth, survival, reproduction and physiological activities and their presence in some tissues of the fresh water fish,, Notopterus notopterus were determined and assessed for the health condition of the fish in the aquatic body from where the fish was collected and reported in the present report. The fish, N.notopterus is available in large numbers in the aquatic bodies of Kalaburagi area. The availability of a particular fish species in wild in large numbers reflects that the fish is thriving well in the local aquatic environment. This fish is being consumed as food fish in the local rural area. The fish feeds on insects, small fishes, crustaceans, young roots of aquatic plants and is considered as corni-omnivorous. This fish is a bottom feeder and comes to the surface for gulping air. The fish N. notopterus feeds by sight and mouth taste and hence brain centre of this fish concerned with vision and taste which are well developed. The fish N. notopterus has prominent optic lobes, facial tube as well as large vagal lobes as found in other fishes. The fish N. notopterus has well developed facial lobe and is capable of recognising food as observed during feeding under laboratory condition, it seems to be possible that the fish exhibits proper correlation of tactile and gustatory impulses and good gustatory sense. The condition of the fish including the length of the fish varies from 21 to 25 cm, and lays eggs from 1200 to 3000, spawning occur at night and this fish relished both in fresh and dried state and has high economic importance as food fish in South and Southeast Asia. The biochemical nutritional components such as protein, glycogen, fat (lipid) and cholesterol and their requirement for the fish to live healthy were reported in some species of fresh water fish and these data were compared with our data for the tissues such as muscle, liver brain and blood of the fish, Notopterus notopterus to assess health condition of the fish in a specific aquatic environment. The results indicated that all the biochemical nutritional compositions were normal at required level in the tissues studied, suggesting that the fish is able to get required nutritional compositions from the natural environment for keeping proper health conditions and thriving well in the aquatic body from where it is collected.