Lionotis nepetifolia (L.) R. Br., is one of the wild members of the family Lamiaceae. The plant has been known for ages for its anti-cold, anti-phlegm, anti-inflammatory and anti-diarrheal properties and is used as ethnomedicine by local tribal communities. The present study is an attempt to investigate the initial phytochemical composition of this plant. The results reveal the presence of bioactive components containing alkaloids, flavonoids, phenolics, tannins, glycosides, steroids, and saponins in various solvents. The medicinal potential of this plant can be correlated with the presence of these phytochemicals.