Titanium alloys have been used in biomedical applications because of their biocompatibility and mechanical properties. However, it has a possible toxic effect resulting from released vanadium and aluminium in the human body. The hydroxyapatite HAP coated Ti-6Al-4V alloy prevented releasing of undesirable ions. The aim of this study is to investigate the structural and mechanical properties of Ti-6Al-4V alloy by coating with (HAp). The coating was prepared by Radio Frequency Plasma Sputtering (RF) method. The layers of HAp were characterized by Field Emission- Scan Electron Microscope (FE-SEM), atomic force microscope (AFM) and Vickers hardness respectively. AFM and the Vickers hardness demonstrated different roughness and hardness of Ti-6Al-4V when uncoating, as-deposited HAp.