The present investigation is carried out in Vasai – Virar Municipal area, which has varied geographical structure. Climate is comparatively humid and temperature is variable. Extramural aerobiological research includes aero microbial survey at various places of Vasai, Nalasopara and Virar region. The aero mycological survey was carried out from June 2017 to May 2018 by using Petri-plate exposure method and Gravity slide sampling method. Total Twenty six fungi trapped from the air. This study is very important with respect to qualitative and quantitative information about the airborne fungi. The prevalence of dominant airspora was found to be correlation with the meteorological parameter like Temperature, Humidity and Humidity. The maximum mycoflora was registered during the monsoon period between Julys to October, which is gradually decreased during summer from March to June. The significant fungal spore type includes species of Alternaria, Bispora, Aspergillus, Albugo, Chaetomium, Cladosporium, Curvularia, Epicoccum, Fusarium, Helminthosporium, Hypoxylon, Humicola, Mucor, Nigropsora, Pleospora, Periconia, Penicillium, Tetrapola, Torula, Smut, Rust etc.