Biotin binding proteins like avidin and streptavidin bind to dietary biotin, making it unavailable to the insects, which then die from a deficiency of this vitamin. Avidin is found in chicken egg white and streptavidin is present in culture supernatant of Streptomyces avidinii (Stapley). When these biotin binding proteins were fed by mixing with flour against T. castaneum and C. cephalonica, dose dependent changes in growth parameters was observed. Avidin @ ≥ 10 ppm and streptavidin @ ≥ 7.5 ppm were found to be effective against T. castaneum with zero development period, adult emergence and growth index and in case of C. cephalonica, avidin ≥ 25 ppm and streptavidin ≥ 10 ppm recorded adult emergence, growth index and development period as zero.