Chemical composition, anthelmintic and antimicrobial activity of the essential oil of the aerial part of Cleome rutidosperma
Frank NI Morah, Gloria C Apebende
Background and Aim: Cleome rutidosperma is extensively employed herbal medicinethe aim of this study is to determine the chemical constituents, anthelmintic and antimicrobial activity of the essential oil from the aerial part of Cleomerutidosperma. Materials and methods: Fresh aerial part of Cleome rutidosperma was ground and steam distilled to get its essential oil. Gas chromatography was used to separate the constituents of the essential oil while the individual constituents were identified by mass spectrometry. Antimicrobial sensitivity test for the essential oil was carried out using agar disc diffusion method while minimum inhibitory, minimum bactericidal and minimum fungicidal concentrations were determined through broth dilution method. 1%, 5% and 10% solutions of the essential oil were used for determination of its anthelmintic activity against Lumbricus terrestris. Results: The essential oil contains forty eight natural products which are being indentified for the first time in Cleome rutidosperma. The oil exhibited antimicrobial activity against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Streptococcus faecalis, Candida albicans and Aspergillus niger. Conclusion:The present study further shows that the essential oil has anthelmintic activity against Lumbricus terrestris.