A descriptive study was conducted to assess the knowledge regarding cold chain maintenance among B.Sc. Nursing students in Bombay Hospital College of Nursing, Indore. Objectives are: to assess the level of knowledge regarding cold chain maintenance among B.Sc. nursing 2nd year students and to find out the association of knowledge scores regarding cold chain maintenance with their selected socio-demographic variables. 10 B.Sc. Nursing 2nd year students were selected by using probability simple random sampling technique with lottery method. Data were collected through socio-demographic variables & structured knowledge questionnaire tools. The results showed that mean score of knowledge level was 9.5 & this value indicate that B.Sc. Nursing 2nd year students were having average level of knowledge regarding cold chain maintenance and there was significant association between knowledge scores with selected socio demographic variables.