In the present investigation an attempt has been made to evaluate the antagonistic activity of Trichoderma against root rot fungi of Soybean, eight different fungi like Fusarium solani, Macrophomina phaseolina, Fusarium oxysporium, Phytopthora sojae, Fusarium moniliforme, Fusarium roseum, Rhizoctonia solani, Sclerotium rolfsii were tested. Trichoderma retarded the growth of Fusarium moniliforme completely (85 mm) followed by Rhizoctonia solani (81.5 mm), Fusarium oxysporium (80 mm), Fusarium solani (79 mm), Fusarium roseum (79 mm), Phytopthora sojae (76 mm), Macrophomina phaseolina (73.5 mm). Minimum growth of inhibition is shown against Sclerotium rolfsii (61 mm).