An experiment was conducted during the year 2016 at Shri Vyankatesh Arts, Commerce & Science College, Deulgon Raja, Buldhana (MH) India, to study the impact of physical treatments of seeds with GA3 ( i.e. Control, 700, 710, 720, 730, 740, 750, 760, 770, 780, 790 and 800 ppm concentration) on seedling growth in Annona squamosa. Seeds physically treated with of GA3 at 790 ppm significantly enhanced the plant height (41.66cm), number of bipinnate leaves (8.33), maximum root length (28.66 cm), seedling fresh biomass (6.59 g) and dry biomass (2.55g) at 45 Days after sowing (DAS). Hence, it may be concluded that seeds pre- treated with GA3 at 790 ppm play an important role for obtaining better quality seedlings of Annona squamosa.