Metals with normal concentration have essential roles in body metabolism, however in higher concentration they can be induce sever toxicity. Treatment with chelating agents is useful practice to reduce metal toxicity in live organism. The leaves of Indian Guava (Psidium Guajava) were taken with the aim of evaluating the chelating activities. The extract in three different solvents viz. aqueous, methanol and petroleum ether were prepared. The ability was studied by using Benzie and Strain method in EDTA was used as standard for this purpose. Methenolic extract was found to be most effective iron chelator. Highest activity was found in 1000µg/ml concentration of guava leaves i.e. 68.20% that of standard EDTA was found to be 89.00%. In conclusion the methanolic extract of Indian guava (psidium guajava) could be used in treatment of iron overload diseases.