Background: Blood stream infection causes significant morbidity and mortality in all age group and it results in increase health care expenditure Blood culture remains main stay for laboratory diagnosis of blood stream infections Aim of the study is to know the bacteriological profile and antibiotic susceptibility pattern of bacteria causing blood stream infections in pediatric age group.
Material & Method: Present study has been conducted between August 2017 to September 2018, all blood samples received for culture & sensitivity from pediatric ward suspected of having sepsis were included in the study .Blood samples were collected using aseptic precautions. Both blood culture bottles were incubated at 370 C for 10 days and subcultures were done on alternate day on blood ager and Mac Conkey medium. In case of growth, organisms were identified using standard protocols and antibiotic sensitivity testing was done using Kirby bauer disc diffusion method.
Results: Total blood samples received between August 2017 to September 2018 were 1604 out of which 395 samples were from Pediatric age group. Growth was seen in 146 (35%) samples. Most common organism isolated from blood cultures from grow positive bacteria spectrum were Staphylococcus aureus were 72 (49.3%) followed by Enterococcus faecalis 6 (5.5%) and Streptococcus pneumoniae 2 (5%).Antibiotic sensitivity pattern showed 100% sensitivity to Vancomycin, Linezolid, and highest resistance was noted for penicillin and tetracycline. Among gram negative isolates most commonly isolated was Klebsiella pneumoniae 20 (13%), followed by Acinetobacter baumanii 20 (13%), Pseudomonas aeruginosa 14 (9.58%), E coli 8 (5.5%) Enterobacter aereogens 4 (2.73%).Overall antibiotic sensitivity pattern shows 100% sensitivity to Colistin followed by 60% for Imipenem, Ciprofloxacin 56%, Piperacillin Tazobactam 50%, Amikacin 46%, Ampicillin 30% and Cefotaxime 26%, Males 84(57%) more commonly affected then females 62 (42%). Most common age group affected was less than 1 year (66/146) 45% in both sexes followed by equal number of cases in age group 2-6 years and 7-12 years in both males and females 40 (30%) respectively.